Single-Molecule Fluorescence Imaging
A cutting-edge technique that captures the real-time dynamics of individual molecules, providing unparalleled insights into genome maintenance, transcription regulation, and molecular interactions.
Applications in Genome Maintenance and Transcription Regulation
Key discoveries about how enzymes maintain genome integrity and regulate transcription at the molecular level, uncovering mechanisms previously hidden in ensemble data.
Development of Molecular Diagnostics
A transformative approach to cancer detection using single-molecule fluorescence to identify biomarkers with exceptional sensitivity for early diagnosis and personalized medicine.
Single-Molecule Manipulation Techniques
Innovative tools like optical and magnetic tweezers that enable precise control of biomolecules, revealing the mechanical properties and dynamic behaviors of molecular systems.
Frequently Asked Questions
What is single-molecule fluorescence?
A technique that visualizes and tracks individual molecules in real-time, revealing molecular behaviors and interactions at unprecedented detail. It provides insights into dynamic processes often masked in bulk experiments.
What is FRET?
FRET (Fluorescence Resonance Energy Transfer) is a spectroscopic technique that measures nanometer-scale distances between fluorescent molecules. In single-molecule studies, it acts as a molecular ruler to detect conformational changes in biomolecules.
How does single-molecule FRET reveal enzyme mechanisms?
It allows observation of individual enzyme molecules during catalysis, showing real-time conformational changes, binding events, and reaction steps. This reveals hidden intermediates and diverse behaviors of enzymes.
How is it used in molecular diagnostics?
Single-molecule fluorescence enables detection of rare biomarkers or genetic mutations at extremely low concentrations. This high sensitivity is crucial for early disease detection, treatment monitoring, and developing personalized medicine approaches.
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physical biology, enzymology, molecular diagnostics